Overview
Adequate mechanical stimulation is essential for cellular health and tissue maintenance, including articular cartilage, which lines the articulating bones in joints. Chondrocytes, which are the sole cells found in articular cartilage, are responsible for matrix synthesis, maintenance and degradation. It is generally believed that chondrocytes require mechanical stimuli through daily physical activity for adequate cartilage homeostasis. However, to date, the molecular mechanisms of cellular force sensing (mechanotransduction) are not fully understood. Among other mechanisms, the cytoskeleton is thought to play a key role. Despite that gravity is a very small force at the cellular level, cytoskeletal adaptations have been observed under altered gravity conditions of a parabolic flight in multiple cell types. In this project, we developed a novel hardware which allowed to chemically fix primary chondrocytes at multiple time points over the course of a 31-parabola flight. The samples were subsequently stained for the cytoskeleton network and microscopic images were acquired. The images showed a large heterogeneity among the cells in morphology as well as in the structure of the networks. In all, no changes or adaptions in cytoskeleton structure could be detected over the course of the parabolic flight.
